We have a long experience in synthesis of a variety of libraries ranging between focused libraries 10-30 members to large 2000 member libraries. The dedicated library synthesis team and an allied infrastructure with unidirectional flow of job from synthesis to purification to evaporation to dilution and to final QC makes the process error free.
Our capabilities include synthesizing libraries in both solution and solid phase, synthesis of scaffolds/building blocks including complex structure involving multi-step synthesis in milligram to kilogram scale with complete characterization, lead generation, lead optimization and SAR libraries generated in 5-50 mg scale with >90% purity and LCMS/UV/ELSD data for each library in customer preferred format including vials/shallow/deep/well MT plate.
Types of Chemistries Handled
Spirocyclization using beta-diketones, Suzuki coupling using variety of reagents, Nuceophillic and SNAr type aminations, amidation and esterifcation, acylation, alkylation, urea, carbamate, pyrazole, pyridone, spiroisoxazoline, oxadiazole formation, reductive amination, ether formation for large libraries, peptide libraries and solid phase amindation.
Reaction Parameter Variability
Solution phase parallel synthesis at operating temperature range of -20oC to 150oC and in inert atmosphere (when needed).
24 wellplate to 86 wellplate formats on flat bed and orbital rotary type shakers. Custom designed teflon filtration.
Purification and Characterization
Laboratory scale purification through normal/reverse phase Biotage Quad 3 systems, Combiflash systems, ABI Q trap/API 2000/Water's analytical LCMS, Water's and Agilent auto purification systems (DAD/Mass detectors), and analytical HPLC/UPLC systems (UV-DAD/ELS) detectors.
Evaporation techniques include vacuum centrifuge, freeze dryer apparatus and shelf freeze drying for higher throughput.
Identification of the pure fractions by FIA-MS analysis, purity of fractions established using UPLC and the pure compound further analysed by LCMS.